Abstract: With the increasing development of bacterial resistance to antimicrobial, Antimicrobial Susceptibility Testing (AST) had became an important and indispensable step for guiding reasonably therapy in clinic. So the perfection and development of AST methods were the premise of the acquirement of the scientific and accurate bacterial drug resistant spectrum, and also were the guarantee of the effective treatment in clinic. In order to provide reference materials for the development and perfection of AST methods according to the characteristics of application in different industries, the traditional AST methods were introduced, such as disk diffusion method, dilution method, Etest method and so on. Their characteristics, advantages and disadvantages were briefly analyzed, and the new and fast detection methods in recent years were concluded. In addition, the development and application trend of the AST method were also prospected.