Abstract: Explants of ‘dwarf Yunong rootstock No.1’ stem segments were cultured in vitro to select the most suitable initial culture. The MS was selected as the basic culture, plus different contents of IBA and 6-BA. By screening of different complete medium, and seeking the suitable initial culture which could improve germination rate of ‘dwarf Yunong rootstock No.1’, to establish the basis for peach ‘dwarf Yunong rootstock No.1’ rapid propogation system. The results showed that: the most suitable initial culture in the fast reproduction system was MS+1.6 mg/L 6-BA+0.4 mg/L IBA, and the bud induction was rising with the increase of the contents of hormone in the scope with IBA 0.1-1.0 mg/L, 6-BA 0.8-1.6 mg/L. In a word, the most suitable medium for the bud induction of stem segments was MS+1.6 mg/L 6-BA+ 0.4 mg/L IBA.