Abstract: Maturity stage is subcritical period of water demand for flue-cured tobacco. This study was to demonstrate effect of different soil moisture regimes at the maturity stage on content of total sugar, reducing sugar, starch, total nitrogen and alkaloid in flue-cured tobacco in field. The results showed that: with soil moisture content of 70% to 85%, the flue-cured tobacco had high contents of tobacco total sugar, reducing sugar and starch at the late growing stage (after transplanting approximately 83 d); drought stress decreased carbon metabolism, however, after the drought re-watering enhanced carbon metabolism. When soil moisture content of 70% to 85%, total nitrogen and nicotine levels was lower; drought stress promoted nitrogen metabolism, and total nitrogen and nicotine content was increased; while re-watering after drought stress, contents of tobacco leaf nitrogen and nicotine was in the middle level. At the maturity stage of tobacco, soil moisture was too high or too low, not to facilitate the coordination of the balance between tobacco leaf carbon and nitrogen metabolism.