Abstract: Pistacia chinensis Bunge is a superior species for biomass energy, however, its seeds contain endophyte, which is a serious problem for tissue culture. In order to solve the issue, different seed sterilization approaches, such as disinfectant types (NaClO, HgCl2, H2O2), disinfectant time and methods were studied. The results showed that: the treatment with 20% NaClO or 0.1% HgCl2 solution could effectively eliminate the endophyte, but these treatments had a negative effect on seed germination. The germination rate was up to 46%. The contamination inhibition rate was significantly decreased and the seed germination was improved when 10% H2O2 was used as disinfectant. Much better effects happened in the treatment with 20% NaClO solution followed by ultrasound for 10 min, which was efficient for eliminating the seed endophyte and also helpful for the seed germination. The germination rate reached 79%.