Abstract: In order to make further comprehend of genetic basis of Spring Wheat Varieties in Xinjiang, and provide some fruitful reference for spring wheat breeding work, this paper analyse the genetic background of 24 Spring wheat varieties Using SSR.The results showed that: 432 alleles were detected by 41 pairs of SSR primers, polymorphic loci accounted for 93.1%, polymorphism information content (PIC) average to 0.86; cluster analysis on genetic distance of 0.8, provided materials can be divided into 4 categories, the species have relatives does not necessarily can be clustered into one group, it indicated that there was rich genetic diversity among spring wheat varieties.