Abstract: For screening Antagonistic organism against Sclerotinia sclerotiorum efficiently, 74 soil samples of Heilongjiang Province were collected, isolated through dilution plate method and primarily screened through confronting incubation on PDA plate. The result showed that 38 antagonistic organism against Sclerotinia sclerotiorum were isolated and screened from 303 strains isolated from 74 soil samples. In the test of antagonistic strains on the germination of sclerotia and on detached leaves, M1M2 was found efficiently suppressing Sclerotinia sclerotiorum with more than efficacy of 90%. The efficacy of 83.52% was obtained in biocontrol test in the greenhouse by treating soil with 100ml fermented liquid. So M1M2 was a prospect strain with the value of further research and application.