Abstract: Self-reproducing bulb-lets of eastern lily “Siberia” (perimeter 4-6cm) as materials, selected bulb-lets reproducted by meristem, bulbel, cuttage and test-tube, studied on refrigeration physiological changes and activity at 2 ±0.5℃. The results showed that: soluble sugar content, soluble protein content and nitrate reductase activity of different type of lily bulb-lets all had the bottom and jump height during the cold storage, bulb-lets reproduced by meristem, bulbel and cottage had the similar changes, physiological changes of test-tube bulb-lets were obviously lagging behind a period. Test-tube bulb-lets had high moisture content, water lossed quickly, and the respiratory intensity was high during the cold storage, out of the cold storage, the germination rate and vigor index of test-tube bulb-lets were lower than bulb-lets reproducted by meristem, bulbel and cuttage.