Abstract: In order to solve the problem of the shortage of Erigeron breviscapus resource, endophytic bacteria in plants and soil of Erigeron breviscapus were isolated and identified. Endophytic bacteria were isolated and identified by using pure culture and 16S rDNA morphology. 100 endophytic bacteria were isolated from Erigeron breviscapus. The numbers of bacteria strains from plant and soil were 62, 38 respectively. Bacillus and Pseudomonas were dominant endophytic bacteria in Erigeron breviscapus and soils accounting for 55% and 28% of total endophytic bacteria. The results showed that quantity, species, and distributing of the endophytie bacteria in different part of Erigeron breviscapus and soils are different, the quantity of Endophytic bacteria from leaves and normal soil were the highest.