Abstract: For germplasm conservation and rapid propagation of Pterocarya stenoptera C.DC., the stem-segment with axillary buds taken as explants to be studied for tissue culture system. The results showed that, the best sterilization method was that explants could be treated with 75% ethyl alcohol and 0.1% HgCl2 for 30 s and 5 min, respectively. The percentage of sterile explant could arrive at 87.5%. The best basic medium of callus proliferation was MS, but DKW was better than MS and B5 to be used in test-tube plantlet multiplication and root induction. It was suggested that DKW supplemented with (1.0-2.0) mg/L 6-BA+1.0 mg/L KT+(0.3-0.5) mg/L IBA to be desirable treatment for subculture and multiplication, with 4 times multiplication rate after a 20-25 days cultivation. The media with 1/2 DKW+(0.5-0.7) mg/L NAA or 1/2 DKW+0.5 mg/L NAA+0.2 mg/L IAA was suggested for the rooting induction. The rooting rate was above 82%.