Abstract: The effects of crude toxin on young embryo culture and scab-resistance of their offspring in R1 and R2 generations were investigated with three wheat varieties. The results were as follows：①High concentration （6.0g/L）of crude toxin inhibited the growth of calli from different genotypes obviously，but the germination and growth of embryos were promoted. When the concentration was lower than 6.0g/L，the crude toxin promoted the growth of calli. ②The crude toxin inhibited the formation of embryogenic calli and regeneration of plantlets remarkably，and the influencing degree was much stronger to former than to later. ③The appropriate concentrations of crude toxin stress were 1.5g/L~4.5g/L，in this range，the calli grew normally，and embryogenic calli and plantlets were inhibited markedly，but their differentiation capabilities were retained in a certain degree. ④The selection of toxin increased the proportion of resistant plants in the R1 offsprings. Scab-resistance of R2 regeneration separated, and there was a tendency of susceptible in the separation.