Abstract: In order to explore and study the application of GIS system in ecological areas, especially the prominent role in which testing of remote sensing technology played in ecological function regionalization. The basic data of Mishan City, Heilongjiang Province administrative districts as the research object, by using in July 2012, 133/28, 133/29, 134/28, 134/28, 133/29 5 TM satellite data and basic topographic maps, the author set introduces overall study area. By using ERDAS correction, image enhancement processing of satellite data, combined with the basic status data, the author conducted a supervised classification. The results showed that: supervised classification was divided into 4 types: forest, grassland, cultivated land and waters, and established the classification of satellite image interpretation. Using the spatial analysis method and combining with the principle of ecological function zoning, the ecological function regionalization was carried out on the basic data, and Mishan City were divided into 4 ecological functional areas: Ⅰ northwest forestry ecological construction development area; Ⅱ industrialized and intensive development area along the railway; Ⅲ south-central agriculture construction development area; Ⅳ Xingkai Lake ecological tourism and fishery development area.