Abstract: In order to reveal spatial heterogeneity pattern of surface mineral soil carbon and macro-nutrients in subtropical evergreen forests, the author investigated carbon and nutrients variability of surface mineral soil (0-10 cm) in subtropical evergreen broad-leaved forest in Ailao Mountains, and employed grid-sampling method to collect soil samples and found that spatial variability of soil total phosphorus, exchangeable calcium, exchangeable magnesium revealed constructive variation and the effective range of these three variables approach 300 m. The models of semi-variogram of soil total carbon, total nitrogen and exchangeable potassium were linear models, which meant that spatial variation of these three variables was non-constructive stochastic variation. It was concluded that: part of soil nutrients in the subtropical evergreen broad-leaved forest exhibited spatially constructive variation on the present sampling scale.