Abstract: To explore the influence of different ecological, variety and interaction between its ecological and varieties on flue-cured tobacco main economic characters, five varieties, HB023, HB061, HB086, HB107, HB112, and normal controls‘( Yunyan 87’and‘K326’) were chosen and planted in Xiaoguang, Liangwu, Yerengu, Huangliang and Maliang in Hubei Province to analyze the influence of interaction between its ecological and varieties on flue-cured tobacco yield, output value, the proportion of the superior and medium tobacco. The results showed that the economic characters of tested varieties in five test sites were higher than normal controls, the difference between the pilot area for significant economic character index and different, especially in Baokang pilot area, the tested strains of output, output value, the proportion of superior tobacco smoke and medium scale significantly higher than those of‘Yunyan 87’and‘K326’clouds that Baokang zone pilot ecological condition to improve the economic benefit was very advantageous; flue-cured tobacco HB023 and Fangxian County area pilot, HB086 and Xuan’en zone pilot, HB112 and Xing Mountain and Fangxian County area pilot interactions, its yield and output value, the proportion of superior tobacco evidently higher than those of control‘Yunyan 87’and‘K326’; HB061 and Xuan’en zone pilot interactions, the output value and the proportion of medium smoke relative to control were improved. HB107 and Fangxian County area pilot interactions, the yield increased with contrast that ecology and species interactions to improve the economic benefits of new strains tested.