Abstract: A field experiment was conducted to study the nitrogen application rate and remained leaf number on yield and main quality traits of flue-cured tobacco with HY-9-7 as material. The results showed that (1) the yield of flue-cured tobacco significantly increased with the increment of nitrogen rate, and it showed the increase-decrease trend with the increment of remained leaf number, and it showed 20 leaves>18 leaves >22 leaves. The output value of flue-cured tobacco increased and then decreased with the increment of nitrogen rate and remained leaf number. (2) As the increase of nitrogen application, total sugar and reducing sugar content decreased, while N, K, nicotine content of cured leaf increased. (3) As the increase of remained leaf number, content of total sugar, reducing sugar, K and nicotine were decreased. (4) The interaction effects of nitrogen rate and remained leaf number on yield, output value of flue-cured tobacco and content of total sugar and reducing sugar in cured leaves were significant.