Abstract: In order to increase the utilization rate of waste banana stalks, the research applied banana stalks as compost feedstock and cellulolytic bacteria as an inoculum under simulated industrial manufacturing circumstances. The effects on the temperature alteration, organic matters decomposition, microorganism number, and coarse fibers degradation were studied. The result indicated that the cellulolytic bacteria not only raised the temperature of compost and prolonged the high temperature phase, but also facilitated the decomposition of organic matters in composting, rendering 33.71% decline of organic content. The inoculum of cellulolytic bacteria could quickly increase the total number of microorganism in early compost and improve the diversity of bacterial community, therefore accelerating the overall composting process. The total degradation rate of coarse fibers reached 33.69% when inoculated with cellulolytic bacteria, which was a 15.58% increase compared to control sample.