Abstract: 56 Euonymus fortunei clones ,3 Euonymusclones and 1 Euonymus clone were analyzed with RAPD. Using 35 arbitrary primers, we scored a total of 205 stable, clear and repeatable DNA fragments. Using 32 of these primers we scored 100 polymorphic bands. The average polymorphism rate was 48.78%. Different primer and different location revealed significantly genetic difference. The range of genetic diversity index (GDI) was from 0.0331 to 0.8904.Through the RAPD bands revealed by primer L04 and U12 and the cluster analysis of genetic distance we can obviously differentiate Euonymus fortunei and Euonymus, defoliate, not defoliate and partly defoliate type of Euonymus fortunei in winter, the breed collected from our country and the 3 breeds introduced from Czechic and America . Cluster analysis can also differentiate the Euonymus fortunei resources collected from southeast littoral and other areas of our country.