Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (2): 39-44.doi: 10.11924/j.issn.1000-6850.2013-1619

Special Issue: 生物技术

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Establishment of S Phage Synchronization system for BmN Cells

  

  • Received:2013-06-13 Revised:2013-07-11 Online:2014-01-15 Published:2014-01-15

Abstract: S phase of cells is the DNA replication phase, exploring the synchronization of S phase will build foundation for the studies of various factors on the regulation of DNA replication, cell division and proliferation. In this work, three methods (TdR double occlusion, TdR-Nocodazole double occlusion and hydroxyurea occlusion) were used to study the S phage synchronization efficiency of BmN cells. The results showed that S phage sychronization efficiency of TdR double occlusion treatment was 67.2% , TdR-Nocodazole double occlusion was up to 69.7% , while hydroxyurea had the best sychronization efficiency with percentage more than 90%. Furthermore, the detailed conditions of HU occlusion were analyzed. The results showed that the highest S phase synchronization efficiency of BmN were up to 94.9% after 16 h treatment by 1 mmol/mL HU, following by being released in normal medium for 7 h. The results of different methods used to study the S phage synchronization efficiency of BmN cells showed that treatment of 1 mmol/mL HU for 16h and then were released in normal medium for 7h was the best synchronization method for BmN cells with its simple and easy operation.