Abstract: In order to study the mechanism of bulb development, the test material was oriental hybrid lily ‘Sorbonne’. Comparison of different culture methods in ultra-structure of physiological indexes (such as starch content, soluble sugar content, protein content) and starch granules of oriental hybrid lily was studied. Results showed that: the marketable bulb of starch granules was obvious more than that in cuttage and in test tube in quantity. Water content in the marketable bulb was the least in the three. The result was consistent with the determination of starch content. Protein content in the marketable bulb was the lowest, which in test tube bulb was highest. The starch granules which were big and small were co-exist in marketable bulb, and that was the same size in cuttage or in test tube. The quantity of bulb of starch granules was obvious the most in the basal than that of the middle and the upper, whether it was marketable bulb, bulb in cuttage and in test tube.