Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2013, Vol. 29 ›› Issue (36): 302-305.doi: 10.11924/j.issn.1000-6850.2013-1709

Special Issue: 生物技术

• 23 • Previous Articles     Next Articles

Transformation of Arabidopsis thaliana with Haloxylon ammodendron HaPrxQ Gene

  

  • Received:2013-06-21 Revised:2013-07-24 Online:2013-12-25 Published:2013-12-25

Abstract: HaPrxQ is an important regulatory factor involved in scavenging reactive oxygen species. In this paper, the full length HaPrxQ gene from the genomic DNA of Haloxylon ammodendron was isolated with PCR technique using primers designed according to the published sequence. To identify potential function of this gene in plant, expression vector pCAMBIA2300-HaPrxQ was constructed, and then transformed into Arabidopsis thaliana by Agrobacterium-mediated in florescence infection method. T0 transgenic seeds were screened by MS plate containing Kanamycin. 9 positive transgenic seedlings were identified by PCR, providing material for further studies on the function of HaPrxQ gene and its interaction with other genes in plants.