Abstract: Effects of pyroligneous application on yield and quality of organic tobacco were studied by field plot experiments. The influence and role of treatments with root pouring fertilizer and foliar-spraying dilute solution on organic tobacco grows and quality was compared in this study. The results showed that the application of pyroligneous increased the yields and production value of organic tobacco and treatments with 150 kg/hm² root pouring fertilizer and spraying of 1000 times diluted solution were relatively better. The yield was increased by 32.0% and 25.4% while the production value was increased by 52.0% and 32.4%. However, vary of organic tobacco stem diameter was not significantly with application of pyroligneous, but increasing plant height and maximal leaf area during topping stage. The effect of spraying diluted solution on potassium content of organic tobacco leaves was superior to root pouring fertilizer, spraying of 1000 times diluted solution was the best treatment which increased the potassium content by 1.44%. The total sugar, reducing sugar and nicotine of organic tobacco were moderate and its coordination was better than control. The application of pyroligneous significantly reduced the incidence of organic tobacco mosaic disease and black shank, but had no effect on tobacco powdery mildew.