Identification of Rice Endophytic Strain K12G2 and Research on Plant Growth Promoting Traits

doi:10.11924/j.issn.1000-6850.2013-1877

Abstract

Abstract: In order to exploit new plant growth promoting microbial fertilizer to replace chemical fertilizer and pesticide, an endophytic strain K12G2 which was isolated from rice seeds was identified and its plant growth promoting action was tested by plate cultivation and water planting. Based on morphological, physiological, biochemical and molecular identification, strain K12G2 was belonged to Curtobacterium citreum. Strain K12G2 was capable of IAA production, phosphate solubilization, nitrogen fixing and starch decomposition. Under gnotobiotic culture conditions, rice seeds treated with strain K12G2 for 1 hour showed more effective than seeds treated with 4 hours. Strain K12G2 exhibited significant increases in radicle and plumule elongation of inoculated seeds under gnotobiotic conditions, and root length (47.08%) of rice seedlings by water planting. Furthermore, rate of three-leaf stage seedlings was 4.5 times that of control. This study provided a good experimental strain for further industrial utilization on plant growth promoting.

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