Resistance Analysis of the Rice Cultivar ‘Lianjing 7’ to Rice Black-streaked Dwarf Disease

doi:10.11924/j.issn.1000-6850.2013-2161

Abstract

Abstract: To analyze the resistance of‘Lianjing 7’against rice black-streaked dwarf disease, and find the linked marker with the resistant gene, the resistance analysis of‘Lianjing 7’, its parents and the F_2:3 population, which derived from a cross between‘Pei’ai 64’, a susceptible variety, and‘Lianjing 7’, a variety with resistance to RBSDS, were evaluated using natural infection methods and artificial inoculation by scoring the disease rating. The result showed‘Lianjing 7’was resistant to RBSDD with the disease rate between 5.5% and 12.25% in all test. In the parents of‘Lianjing 7’, only‘Zhongjingchuan-2’was resistant to RBSDD. The antixenosis and antibiosis score of‘Lianjing 7’was similar to the susceptible variety‘Wuyujing 3’, so the resistance of‘Lianjing 7’was mainly caused by the resistance to the RBSDV. In the Lianjing 7/Pei’ai 64 F_2:3 population, the disease rates showed a continuous distribution with transgressive segregation, it indicated the resistance of‘Lianjing 7’was controlled by polygenic. Finally, we got the marker RM287 closely linking with the resistance loci by BSA. To sum up,‘Lianjing 7’was resistant to RBSDD, and it acquired the resistance of‘Zhongjingchuan-2’. The marker RM287 should be useful for breeding new RBSDD-resistant cultivars by marker-assisted selection (MAS).

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