Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (17): 180-185.doi: 10.11924/j.issn.1000-6850.2013-2325

Special Issue: 生物技术

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Optimization of Ultrasonic disruption for PAHs-depredating enzymes from Actnetobacter calcoaceticus

  

  • Received:2013-09-02 Revised:2013-10-21 Online:2014-06-14 Published:2014-06-14

Abstract: Ultrasonic wave was used in this paper to disrupt the cell of Actnetobacter calcoaceticus WG-6 for PAHs-depredating enzymes. The effect of ultrasound output power, duration of radiation each time, total irradiation time and the cell dilution multiple on the activity of PAHs depredating-enzymes were investigated through the single-factor experiment. The optimum conditions of PAHs depredating-enzymes extraction were determined through box-benhnken center-united design and response surface method were duration of radiation each time 2s (with a 2s interval), ultrasonic power 320W, total irradiation time 17 min, cell dilution multiple 12.5. The PAHs-depredating enzymes activity was 95.55U/g under this condition.