Abstract: The author built the system of callus induction, proliferation and suspension culture in Fraxinus rhynchophylla. Leaves and petioles germinated from dormant buds of Fraxinus rhynchophylla were chosen as explants using the WPM as the basic culture medium. The effect on the formation and proliferation of the callus regulated by plant auxin of different concentrations and proportions was determined by uniform experimental design. Calluses induced by the combination based on 1.8 mg/L NAA and 0.1 mg/L TDZ were green and loose. 1.2 mg/L NAA with 0.07 mg/L TDZ can be used to form suspended callus cultures with high proliferation rate. The callus inducing medium: WPM+1.5 mg/L NAA+ 0.1 mg/L TDZ. The best medium for callus proliferation: WPM + 1.874 mg/L NAA + 0.127 mg/L TDZ. The best medium for callus suspension culture: 1/2WPM + 1.2 mg/L NAA+ 0.07 mg/L TDZ. The maximum content of aesculin was 0.800 mg/g and it appeared on the 9th day after suspension culture.