The Progress of Plant Transgenic Technology

doi:10.11924/j.issn.1000-6850.2013-2368

Abstract

Abstract: Transgenic technology is a central issue of modern biotechnology, and it is also an important method for gene function analysis and breed improvement. Transgenic technology developed rapidly, and it has widely applied in the field of agriculture, medicine, foods, biology and energy. In order to promote the application of transgenic technologies in extractive industry, genetic transformation techs were systematically reviewed in this paper. Firstly, conventional transformation methods, such as agrobacterium- mediated transformation and ployethylene glycol (PEG) mediated transformation, were summarized; Secondly, receptor systems used for genetic transformation were introduced; Thirdly, the construction of expression vector system were illustrated, in this part, the conception of promoter, marker gene and reporter gene were curtly referred. The advantages and limitations of current transformation methods were also concluded. Further, this review introduced some new transformation methods for the advanced investigation of plant science. Lastly, this paper discussed the security problem and solutions of transgenic technology.

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