Abstract: In order to promote development and application of Vaccinium duclouxii and V. fragile in Yunnan, the paper investigated the resource and analyzed the nutrition composition of these two species of Vaccinium L. The results showed that its distribution was most extensive, especially in northwest of Yunnan. The mine element, total sugar, soluble solids, total acid and coarse fibrous of V. duclouxii were obviously higher than V. fragile. Vitamin C of V. fragile was higher than V. duclouxii. The content of protein and amino acid of them was the same. The nutritional composition of two species of Vaccinium L. was even higher and more comprehensive than V. ashei Reade. The value use was assessed.