The Separation Culture and Screening of IAA-Producing Endophytic Bacteria from the Roots of Phalaenopsis amabilis

doi:10.11924/j.issn.1000-6850.2013-2895

Chinese Agricultural Science Bulletin ›› 2014, Vol. 30 ›› Issue (16): 148-152.doi: 10.11924/j.issn.1000-6850.2013-2895

The Separation Culture and Screening of IAA-Producing Endophytic Bacteria from the Roots of Phalaenopsis amabilis

Received:2013-11-05 Revised:2013-11-15 Online:2014-06-05 Published:2014-06-05

Abstract

Abstract: In order to separate indole-3-acetic acid (IAA) producing endophytic bacteria from Phalaenopsis amabilis roots, we investigated the optimal surface sterilization condition of Phalaenopsis amabilis roots, take the separation culture, biological and chemischial characters, and 16S rDNA analysis. The results indicated that, 6 strains isolated by NA medium were Brevibacterium, Burkholderia, Dyella and Paenibacillus. 20 strains isolated by R2A medium were Burkholderia and Dyella. A total of 23 strains were shown to produce IAA from 26 strains. In general, diverse and abundant communities of IAA-producing endophytic bacteria exist in the roots of Phalaenopsis amabilis which highlight the microbial resources represented by interactions between endophytic bacteria and Phalaenopsis amabilis further.

References

[1] 张萍,宋希强.兰科植物内生细菌物种多样性及其促生机理研究进展[J].热带亚热带植物学报,2012(1):92-98.
[2] Wilkinson K G, Dixon K W, Sivasithamparam K, et al. Interaction of soil bacteria,mycorrhizal fungi and orchid seed in relation to germination of Australian orchids[J]. New Phytol,1989,112(3):429- 435.
[3] Wilkinson K G, Sivasithamparam K, Dixon K W, et al. Identification and characterisation of bacteria associated with Western Australian orchids[J]. Soil Biol Biochem,1994,26(1):137- 142 .
[4] Wilkinson K G, Dixon K W, Sivasithamparam K, et al. Effect of IAA on symbiotic germination of an australian orchid and its production by orchid-associated bacteria[J]. Plant and Soil,1994,159 (2):291-295.
[5] Tsavkeiova E A, Cherdyntseva T A, Lobekova E S, et al. Microbiota of the orchid rhizoplane[J]. Microbiology,2001,70(4): 492-497.
[6] Tsavkeiova E A, Cherdyntseva T A, Netrusov A I. Bacteria Associated with the Roots of Epipbytic Orchids[J]. Microbiology, 2004,73(6):710-715.
[7] Tsavkelova E A, Chcrdyntseva T A, Botina S G, et a1. Bacteria associated with orchid roots and microbial production of auxin[J]. Microbiol Res,2007,162(1):69-76.
[8] Tsavkclova E A, Chordyntseva T A, Klimova S Y, et al. Orchidassociated bacteria produce indole- 3- acetic acid，promote seed germination，and increase their microbial yieldin response to exogenous auxin[J]. Arch Microbiol,2007(188):655-664.
[9] Tsavkeiova E A, Chordyntseva T A, Netrusov A I. Auxin production by bacteria associated with orchid roots[J]. Microbiology,2005,74 (1):46-53.
[10] 孙磊,毕晓宝,李潞滨,等.春兰根内生细菌分离培养方法的初步研究[J].河北农业大学学报,2009,32(1):42-46.
[11] 刘琳,孙磊,张瑞英,等.春兰根中可分泌吲哚乙酸的内生细菌多样性[J].生物多样性,2010,18(2):195-200.
[12] 杨娜,杨波.蕙兰根部内生细菌多样性及季节动态变化[J].植物科学学报,2011,29(2):156-163.
[13] 俞婕,赵凯鹏,董飞,等.野生铁皮石斛内生菌的分离及促生作用研究[J].现代农业科技,2010(9):96-97.
[14] 马秀文,和艳会.采用文献计量法分析我国蝴蝶兰研究现状与发展趋势[J].安徽农业科学,2011,39(6):3761-3762,3765.
[15] 东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社, 2001.
[16] Devereux R, He S H, Doyle C L, et al. Diversity and origin of Desulfovibrio species: phylogenetic definition of a family[J].J Bacteriol,1990,172(7):3609-3619.
[17] Aagot N, Nybroe O, Nielsen P, et al. An altered Pseudomonas diversity is recovered from soil by using nutrient- poor Pseudomonas- selective soilextract media[J]. Appl Environ Microbiol,2001,67(11):5233-5239.
[18] 黄宜莹,高怡婷,徐源泰.兰科植物根部微生物的研究[J].中国园艺(台湾),2005,51(4):347-356.

The Separation Culture and Screening of IAA-Producing Endophytic Bacteria from the Roots of Phalaenopsis amabilis

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