Abstract: In order to investigate the distribution of soil available micronutrients, and provide a theory basis for management of fertilizing reasonably on crops. The contents of major and trace elements (P, K, Ca, Mg, Cu, Fe, Zn, Mn) in farmland soil collected from Yunnan Province were determined by inductively coupled plasma-mass spectrometry (ICP-MS), the contents and distribution of major and trace elements were also studied. The results showed that: the contents of available P, K were balance, available Ca, Mg were deficient, available Cu, Fe, Zn, Mn were very plentiful. Major and trace elements distribution of different landform areas were obvious differences. The contents of trace elements (Fe、Zn、Mn) of central Yunnan Red Plateau were significantly higher than those of Hengduan Mountains in western Yunnan and those of karst plateau in eastern Yunnan. The contents of available P, K, Ca gradually declined from west to east, while the content of available Cu rose gradually from west to east. The results were beneficial to elements determination and agricultural production in farmland soils.