Abstract: 2301S is the thermo-sensitive genic male sterile (TGMS) indica line, which was bred by the Rice Research Institute of Anhui Academy of Agricultural Sciences. To solve the problems existing in the Thermo-sensitive genic male sterile line breeding of rice, this study took 2301S as material, used special ecological advantages of Yunnan with low latitude and high altitude, reproduction test base selection according to the requirements of rice low temperature sensitive genic male sterile line 2301S fertility sensitive stage and flowering stage on the temperature of. This paper studied the effects of different altitudes and sowing date on the reproduction yield and its components of 2301S. For the yield, the results showed that the optimal sowing date of 2301S was different under different altitude conditions. When at the elevations of 1429 m and 1532 m, the theoretical yield of 2301S with sowing date delayed first increased and then decreased, at the altitude of 1429 m, April 24th was the most suitable for planting. But at the altitude of 1532 m, the most suitable sowing date was on April 14th. While at the altitudes of 1631 m and 1663 m, the theoretical yield of 2301S decreased with delayed sowing date, April 4th was the most suitable for planting. By the analysis of variance could be learned, the altitudes, sowing dates and its interactions had very significant effects on the yield and its components of 2301S, such as effective panicle per plant, solid spikelets, seed setting rate and thousand-grain weight. The factors of altitudes and sowing dates had significant effects on the total spikelets of 2301S. The effects of altitudes and sowing dates on total spikelets of 2301S reached significant level, but its interactions effect was not significant. The results indicated that, at the low altitudes of deferred sowing and the high altitudes of early sowing, which was beneficial to production of 2301S. This study could be reference for solving breeding problems of other low temperature sensitive genic male sterile rice.