Abstract: From the viewpoint of ecology, this study explored the effective and economical weeding method in the dry seedling bed under the organic cultivation condition by taking advantage of the biological difference of the rice and weeds. With the Japanese high quality rice variety of‘Koshihikari’as material, the field experiment was done with a ramdomized block design and three replications in 2012, and then the comparative production experiment was followed in 2013. Comparing with the control, the relative rate of weeding in 2012 was T4 (93.6% )＞T2 (89.6% )＞T1 (87.0% )＞T3 (21.1% ); the ratio of yield increase was: T4 (11.0% )＞T2 (10.0%)＞T1 (7.6%)＞T3 (1.0%); the revenue increase was: T2 (4485.0 yuan/hm²)＞T4 (4218.0 yuan/hm²)＞T1 (3534.0 yuan/hm²)＞T3 (330.0 yuan/hm²). The experimental result of 2013 comparing with the control was following: the relative rate of weeding of T2=88.7%, the ratio of yield increase of T2=7.9%; the revenue increase of T2=2562.0 yuan/hm². The result of two years’experiments showed weeding with the method of T2 markedly increased the yield and the revenue. So it didn’t need induct weeds and then destroy them before sowing but sowing the selected dry rice seeds directly and backing the surface soil in time after sowing. This method (i.e:dry seed and backing soil) was an effective and economical way for weeding in dry seedling bed under the organic cultivation condition.