Abstract: In order to investigate the changing characteristics of concentrations for typical anions (Cl^-, NO^3-, and SO₄^2- ) along with the variation of time and water level in water bodies of the Three Gorges Reservoir, Fuling, Shibaozhai in Zhongxian, which are located in the main stream of Yangtze River, and Hanfeng Lake, which is located in the Branch of Yangtze River, were selected as study sites. During the whole year, sections were set in investigate spots for stratified water sampling, and Cl^-、NO^3- and SO₄^2- in water samples were continuously monitored by an Ion chromatography once every two months, with the causes of there change being preliminary analyzed. The results showed that the whole year in research area, ion concentration of SO₄^2-, Cl^- and NO^3- were 14.67±1.71 mg/L, 10.66±4.88 mg/L and 1.89±0.15 mg/L respectively, and they all displayed significant variations along with seasonal change, with a coefficient variation larger than 43%. Meanwhile, the change of water levels at the dam’s reservoir had great influence on the content of anions in main stream. Variations of anion concentration in different sampling sites between Fuling, Zhongxian and Kaixian were quite different, with a coefficient variation larger than 40%. The total ionic concentration of reservoir area in spring, an average of 13.02 mg/L, was much higher than that in other seasons.