A Preliminary Study of“The Three-step Method”in Purification of a Bacteriocin from Lactobacillus paracasei

doi:10.11924/j.issn.1000-6850.2014-0057

Abstract

Abstract: In order to improve the yield and activity of bacteriocin produced by Lactobacillus paracasei HD wy-1, the purification of antibacterial substances was studied with the three-step method. The antibacterial substances were extracted and purified by the Ammonium sulfate precipitation, CM Sepharose Fast Flow ion-exchange chromatography and Sephadex G-75 gel filtration. It is shown that the final yield of bacteriocin was 39.93% and the specific activity was 1.56×103 AU/mg. The specific activity was increased 2.87 fold. Tricine-SDS-PAGE showed that its molecular weight was about 72 KDa. The method had shown good results, which provided theoretical foundation for physicochemical property and development of bacteriocin.

ping jingge. A Preliminary Study of“The Three-step Method”in Purification of a Bacteriocin from Lactobacillus paracasei[J]. Chinese Agricultural Science Bulletin, 2014, 30(24): 316-320.

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