Abstract: The aim was to lay the preliminary foundation for screen drought stress of fiber flax. Flax seeds of 'Tianxin No. 3', YOI303, ' Shuangya No. 5' germination test under PEG stress were engaged by dish filter paper method. Seedlings were obtained after germinated seeds potted. To explore the physical and chemical responses on seedlings to drought stress, seedlings were potted water control experiment. The results showed that the height, root length, chlorophyll content of three species fiber flax seedlings appeared downward trend with increased drought stress, the proline content, soluble sugar content, soluble protein content, CAT activity showed an overall upward trend, but the variation range were different from different species. According to the comprehensive evaluation on drought tolerance, seedlings of YOI303 species was the best, seedlings of 'Tianxin No. 3' and 'Shuangya No. 5' were the second.