Abstract: Through the orthogonal tests and single factor test, the technique of tissue culture and rapid propagation of the leaves of Saintpaulia ionantha was discussed. The result indicated: (1) Initial media:MS+6-BA1.0 mg/L +1BA0.5 mg/L； (2) Clump bud induction media: MS+6-B A1.0mg/L+KT0.1 mg/L + IBA0.1 mg/L; (3) Efficiency seedling induction media:MS+6-BA0.5 mg/L +IBA0.5 mg/L +active carbon 0.3%; (4) The effect was better when cuttage in the out of the bottle was used in rooting of tube seedling .