Abstract: In order to determine the optimal method on fibrinogen extraction, the repeated cold precipitation method, classical cold precipitation method, cold ethanol method, ammonium sulfate precipitation method and polyethylene glycol precipitation method were adopted to extract fibrinogen from pig anticoagulated blood. All methods were assessed according to the blood volume processed, time-consuming and content of fibrinogen obtained in each process, whether additional chemicals need to be added and purity of the product. The results showed that compared with the classical cold precipitation, repeated cold precipitation could significantly improve the fibrinogen concentration. Compared with the chemical method, the whole process is physical, with no need to add additional chemicals, thus do not need further purification. The purity is relatively high and meets the standards of external use of the product. As a conclusion, the repeated cold precipitation method showed the optimal effect in fibrinogen extraction.