Abstract: To understand the relationship of main characters of cotton, 123 cotton breeding materials were evaluated comprehensively. Yield、disease resistance、fiber quality and other characters were correlatively analyzed and fiber quality characters were clustered. The results showed that: (1) the variation coefficients of Fusarium wilt, Verticillium wilt disease index、individual lint yield, seed yield and individual boll number were relatively larger than that of fiber quality characters; (2) there were six significant positive correlation characters with seed yield, in which correlation coefficient of individual lint yield and boll weight were relatively high; the correlation coefficient of upper half mean length with uniformity index was 0.69**, with elongation was 0.78**; the correlation coefficient of elongation with fiber strength was -0.62**, with uniformity index was -0.63**, the maximum correlation coefficient of micronaire value and boll weight was 0.51**; (3) 123 cotton breeding materials were classified into three groups by fiber quality characters. The study provided theoretical basis and reference for breeding material cross combination and cultivating new cotton varieties of high yield、good quality and disease resistance.