Abstract: To make the laying hen manure have the multi-function of preventing soil-borne diseases. We isolated 170 strains from the experimental field and laying hens farms of Anhui Academy of Agricultural. 7 strains were screened out by the plate confrontation experiment carried out to test the inhibition effect of 170 strains to Colletotrichum capsici. After that, we carried out inhibitory spectrum experiments, temperature experiments and proliferation assays with these 7 strains to screen out the effective strains. At last, better ones were taken to molecular biological identification test. The results showed that the inhibitory rates of TCC53 to Sclerotinia sclerotiorum and Rhizoctonia cerealis were 90.4% and 82.3% respectively, the inhibitory rate of TCC157 to Colletotrichum capsici and Phytophthora capsici were 88.6% and 71.2%. And both of them could be grown in more than 40℃. In the molecular biology assay, TCC53 and TCC157 were identificated as Aspergillus aculeatus and Penicillium crustosum. Which were existed in the environment commonly, could grow fast and non-pathogenic, add the 2 strains to the organic fertilizer made by laying hen manure could help the crops resistant disease and improve soil structure.