Abstract: Two media systems were collected to evaluate the percent of callus formation and shoot regeneration from ten potato varieties with their stem as explants. The results indicated: The number ② media system (MS+0.01mg/L NAA+2mg/L BA+5mg/L GA3)is suitable to the most of the ten potato varieties. The most efficient potato varieties is kexin No.16, Its percent of callus formation is 98.74% and shoot regeneration is 90.32%.