Abstract: To establish a method that allowing fast and efficient detection of SRBSDV, a fragment comprising the N- terminal 993 bp of P6 ORF of SRBSDV was cloned into the vector pDEST17 using Gateway recombination technology. Antiserum was obtained by injecting the bacterially expressed peptide to healthy rabbits. Western blot experiments showed that the antiserum could detect P6 specifically in rice plants infected by SRBSDV. The fluorescence antibody could also be used to detect P6 in WBPHs using immunofluorescent labeling technique. These results indicated that P6 antibodies prepared here could be used for efficient and accurate detection of SRBSDV in diseased rice plants and viruliferous vectors by immunofluorescent labeling techniques, which was important for the monitoring and forecasting of SRBSDV occurrence.