Abstract: In order to investigate the change rule of‘Lianjing 7’rice seed germination rate during storage and the effects of the joint action of temperature and water content on rice seed germination rate, 3×3 factorial design was performed with rice seed germination rate as dependent variable, water content and storage temperature as independent variable, and SPSS software was used to process the data. The results showed that the germination rate of rice seed kept in storage with water content of 14.5% and storage temperature of 25℃ for 180 days could still reach 89%, and with water content of 11.5% and storage temperature of 5℃ for 180 days could still reach 96.6%. Rice seed germination rate gradually decreased with the extension of storage time, water content and storage temperature were the key factors and had significant interaction.