Abstract: In order to optimize under film transplanting techniques of flue-cured tobacco in the tobacco rice rotation areas, ‘Yunyan 87’ as material, effects on tobacco growth and physiochemical properties were compared with a light permeable, breathable, waterproof polyethylene transparent woven cloth and ordinary agricultural film. The test results showed that root and stem girth growth of tobacco plant under woven cloth transplanting were faster than ordinary plastic film above or under transplanting tobacco in the root extending stage. Squaring and after topping, the plant height, stem girth and pitch of tobacco plant under woven cloth transplanting were more than them of normal plastic membrane. Weight of single leaf and mass per unit leaf area of middle leaves were larger than them of ordinary plastic film, but the leaf thickness decreased. Total nitrogen and potassium content of tobacco leaf were increased as well as the ratio of nitrogen to nicotine reduction. Under woven cloth transplanting seedling can effectively promote tobacco growing early and fast, increase leaf weight and dry matter accumulation in the Hunan tobacco rice rotation areas.