Abstract: To safely and reasonably use livestock and poultry manures from intensive farming, the field experiment was carried out to study the effects of organic chicken manure application for 2 consecutive years on soil Zn accumulation and wheat Zn uptake. The results showed that after application of 60 t/hm² high Zn chicken manure organic fertilizer for 2-consecutive years, soil Zn content was not surpass the Second Chinese Environmental Quality Standard for soils (GB15618-1995) (pH>7.5, Zn≤300 mg/kg). The total Zn, available Zn contents of soil and Zn content in wheat grain increased significantly with the increasing amounts of the organic chicken manure for 2-consecutive years. Compared with first year of organic chicken manure, the treatment of organic chicken manure application for second year the soil total Zn content increased 1.39%-70.79%, soil available Zn content increased 35.37%-66.25%. There was a significant difference in Zn content in the grain of wheat between control and treatments above 30 t/hm². When organic chicken manure application amount was 60 t/hm², the treatment of organic chicken manure application for second year Zn content of wheat straw and grain increased by 14.75% and 2.74% than for first year. Along with the increasing of total Zn content of soil, Zn content in wheat grain increased significantly. The relationship between the amounts of the contents of soil total Zn and wheat grain Zn could be simulated by mathematic curve for 2 consecutive years. Test results suggested that both correlative coefficient attained very significant level. When the soil Zn content would not surpass the Second Chinese Environmental Quality Standard for soils, for first and second year of chicken manure application, Zn content in wheat grain were 93.21 mg/kg and 100.37 mg/kg, respectively.