Abstract: This study was conducted to screen antagonistic bacteria against Phytophthora parasitica var. nicotianae from tobacco rhizospheric soil and to select compound antagonistic bacteria with high inhibitory effects for biocontrol of tobacco black shank. Soil samples were collected from tobacco fields in Bijie City, Guizhou Province and Luzhou City, Sichuan Province. Plate dilution method was used to isolate soil bacterial strains. The isolated strains were screened by plate confront culture method. The compatibility of screened antagonistic bacterial strains was tested by filter paper method to decide the compound bacteria. Further more, pot and field experiments were carried out to study the inhibition and biocontrol activities of single bacterial strains and compound bacteria. Bacterial strains with strong antagonistic activities were identified based on 16S rDNA sequence analysis. A total of 247 bacterial strains were isolated from 17 soil samples, 19 exhibited antagonistic effect on P. parasitica var. nicotianae among them. 6 bacterial strains with good compatibility made up compound bacterial combinations B3B64, B41B57 and L7L47. In the further experiments, B41B57 and L7L47 showed high inhibition effects which reached 75.72% and 77.95%in pot trials and 67.99% and 71.40% in field trials. The compound bacteria increased inhibition effect compared with the single ones. 16S rDNA sequence analysis identified strain B41 as Bacillus subtilis, L7 and L47 as B. amyloliquefaciens and B57 as Pseudomona putida. The compound bacterial strains B41B57 and L7L47 which exhibited excellent inhibition efficacy of P. parasitica var. nicotianae can be exploited as efficient biocontrol agents against tobacco black shank.