Abstract: Phosphoethanolamine N-methyltransferase (PEAMT) is the key enzyme for phosphocholine. A new gene, named SgPEAMT, was cloned by RT- PCR from Suaeda glauca, which was a typical halophyte. Bioinformatics analysis showed that SgPEAMT encoded 494 amino acids. Hydrophobic analysis indicated that SgPEAMT was a hydrophilic protein and located in cytoplasm. SgPEAMT contained two Sadenosylmethionine-dependent catalyzing domain which contained four motifs, respectively. Phylogenetic tree analyses revealed that the amino acid sequence encoded by SgPEAMT gene was highly homologous to PEAMTs from other Suaeda species. Furthermore, SgPEAMT gene expression patterns in response to NaCl and abscisic acid (ABA) in roots, stems, and leaves of Suaeda glauca were investigated using real time quantitative PCR, which indicated SgPEAMT gene probably played an important role in response of salt stress in Suaeda glauca. The research laid a foundation to further study the PEAMT function and salt resistance mechanism in Suaeda glauca.