Abstract: Small brown planthoppers (Laodelphgax striatellus) are agricultural pests and mediators to spread plant virus. In order to provide sufficient insect materials in vivo for correlational research, a method for mass rearing of small brown planthopper in laboratory was presented. We optimized the temperature, light intensity, rearing density and fixed the time of laying eggs, changing seedlings through a series of biological tests. Results showed that temperature of 24-28℃, light intensity of 5000-7000 lx, rearing density of 300-400 SBPH per beaker (nymph), 200-300 SBPH per beaker (3th-4th instar), and 100-200 SBPH per beaker (adults) were the best condition for rice growing, SBPH development, breeding and hatching eggs of each rearing batch. For the consistency of instars between different batches, the times of 48 hours and 8 to 10 days were respectively fixed for laying eggs and changing seedlings. Factors of rearing condition were explicit using this method, and were easy to operate. It was also not limited to space, time, environment and other natural conditions and could provide enough insects at standard experimental age at any time.