Welcome to Chinese Agricultural Science Bulletin,

Chinese Agricultural Science Bulletin ›› 2015, Vol. 31 ›› Issue (21): 107-110.doi: 10.11924/j.issn.1000-6850.casb15010042

Special Issue: 小麦

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Effect of Culture Media on Seedling Rate of Wheat Haploid

Gao Yan, Tang Jianwei, Yin Guihong, Han Yulin, Huang Feng, Wang Lina, Yu Haifei, Li Nannan,   

  1. Lv Yongjun, Zhang Qian, Li Shuncheng, Yang Guangyu, Li Xinping(Zhoukou City Academy of Agricultural Sciences/Wheat Germplasm Improvement Engineering Research Center of Henan Province, Zhoukou Henan 466001)
  • Received:2015-01-08 Revised:2015-02-09 Accepted:2015-02-25 Online:2015-07-28 Published:2015-07-28

Abstract: In order to select the optimal culture medium for the growth of wheat haploid immature embryo and improve the seedling rate, wheat haploid immature embryos derived from wheat × maize cross were inoculated in eight mediums. Each medium was inoculated two materials. Mean values of the two materials were used for data analysis. The frequency of seedling production in eight mediums were 46.07%, 45.90%, 34.11%, 41.15%, 37.99%, 36.91%, 37.70% and 52.51% respectively. The results showed that the optimum culture medium was pH5.8 medium of 1/2MS, 1.0 mg/L KT, 3.0 mg/L PP333, 100 mg/L inose, 5% sucrose and 0.6% agar. In the optimum culture medium, the immature embryo seedling rate was the highest, albino seedling rate was low and the haploid plantlets grew well. The basic culture medium of 1/2MS, added with trace amount of PP333, KT, inositol and high concentration sucrose, can effectively promote the embryo growth.