Abstract: On the basis of field investigation and the theory of ecological suitability evaluation, the author used GIS technology and the method of fuzzy mathematics, selected 8 potential factors which affected Epimedium planting distribution, built ecological suitability evaluation model and researched artificial planting suitability distribution of Epimedium in Gansu Province. The results showed that the suitable planting area of Epimedium was 4.0342 million hm², accounting for 66.07% of the total area of forest land in Gansu Province; the planting areas were mainly distributed in Gannan, Longnan, Xinglong mountain in Yuzhong county and Maxian mountains in the south of Qilihe district of Lanzhou, and Min county, Zhang county and parts of Weiyuan county of Dingxi, Huating county of Pingliang, and along the route of the Guan-Taitong-Kongtong mountain. Through the appropriate division of planting areas, the author provided theoretical basis for cultivating Epimedium from wild germplasm in Gansu Province.