Abstract: The study is to find out the most efficient way for rapid propagation of Arundo donax，which can lay the foundation for its industrial production, application and transformation. Stems with auxiliary buds were used as explants, different concentrations of plant growth regulators were tested to find the most effective protocol for rapid propagation of Arundo donax. MS 4.00 mg/L 6-BA 1.00 mg/L IBA was the most efficient initial medium for the sprouting of auxiliary buds, the germination rate could get to 96.7%; MS 7.50 mg/L 6-BA 1.00 mg/L IBA was the most efficient proliferation medium, and the average proliferation rate was 13.5, while individual proliferation rate reached 16; 1/2MS 1.00 mg/L IBA was the most suitable rooting medium, in which the rate of rooting was 93.8%, the average root number was 6.33. Plants, which were 7 cm high and with roots 1 cm to 3 cm long, were chosen for further acclimatization. The research establishes the rapid propagation of Arundo donax, improves the propagation coefficient and provides evidence for transformation and breeding at molecular level.