Abstract: In order to study the factors effected Ardisia crenata’s flowering and ascertain the relationship between flower bud differentiation and two main environmental factors, temperature and light, the experiment was carried out which used 1.5-2 year old potted Ardisia crenata as material and set different treatments by temperature, photoperiod and light density. The results showed that temperature was a main factor affecting flower bud differentiation of Ardisia crenata. Under treatment with 20-25℃ Ardisia crenata could undergo the whole process of flower bud differentiation, while under the treatments with temperatures 10℃ it could not undergo flower bud differentiation but just maintained the vegetative growth process. When vegetative growth was strong, flower bud differentiation of Ardisia crenata occurred as long as temperature was appropriate regardless long-day or short-day light treatment. It showed photoperiod had no effect on flower bud differentiation and floral induction, but suitable light density was beneficial to the growth and development of Ardisia crenata.