Abstract: In order to make an optimal selection of substrate and cutting facilities, the tender branch cutting experiments of Acer negundo ‘Auratum’ were carried out with three kinds of culture substrates of sand, perlite, mixed substrates (perlite : turf=1:1), and two kinds of cutting facilities of the plastic awning facilities and the double layer greenhouse culture seedling facilities. The results showed that perlite and mixed substrates treatments were both better than sand treatment. Among the three treatments, perlite was the highest in rooting rate of 95.6% and root number with characters of crude and short, and the higher in root activity. Mixed substrates treatment was the higher in root activity with thin and long root, but was low as 78.4% in the rooting rate. Under the plastic awning facilities with the mixed substrates, compared with the low temperature and low humidity environment, the rooting rate, root activity, and root number were decreased significantly in higher temperature and humidity. However, the rooting indexes and the seedling growth indexes showed no difference between the two different cutting times under the double layer greenhouse culture seedling facilities. Pure perlite was the optimal culture substrate with long range of cuttings time in plastic awning facilities. Under the low temperature and humidity condition, the mixed substrates were suitable for tender branch cuttings under the plastic awning facilities. However, tender branch cuttings with the double layer greenhouse culture seedling facilities still could maintain a high rooting rate under the high temperature and humidity condition.